Ptet Promoter Sequence
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Promoter is constitutively on and repressed by tetr.
Ptet promoter sequence. Plasmid r0040 ptetab from dr. This sequence has a bamhi cleavage site sequence in the 3 end. Epub 2015 nov 4. A preferred sequence having two or more restriction sites ie multi cloning sites is described in the sequence id no2.
Germline coding sequence mutations in pten have been shown to cause approximately 35 of cowden syndrome cs a multisystem disorder characterized by increased risks for breast colon endometrial renal cell and thyroid carcinomas. Ptet on vector for expressing the rtta transactivator in the tet on system. Tetr repression is inhibited by the addition of tetracycline or its analog atc. Tetr repressible promoter sequence for ptet inverting regulator.
C the newly designed ptet promoters are shown consisting of a tet operator heptamer with 36nt spacing and randomized fusion points between all the operators. 1 4 although clinical testing usually includes sequencing of the pten promoter most changes are classified as variants of uncertain significance vus which provides no direction for clinical management. Coli employing a synthetic promoter library on ptet partbbar0040. The cmv minimal promoters differ in size but both contain the authentic tata box and initiator sequence inr.
We have improved the ptet promoters function and inducibility for use in e. The spl was constructed using a pcr with randomized primers to yield arbitrary sequences in the promoter region. Douglas densmores lab contains the insert controllable promoter and is published in acs synth biol.
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